Don't miss your email and connect your friends with TalkKing!
We are pleased to present a summary of a recent successful
nanobody
development project, demonstrating our streamlined platform from target to candidate.
Our team successfully generated specific nanobodies against a client-provided peptide target (B-N) using alpaca immunization and phage display technology.
Key
Technical Route
Alpaca Immunization
:
Alpacas were immunized five times with B-N-BSA, and ELISA confirmed steadily increasing serum titers.
Library Construction:
PBMCs were used to prepare cDNA and amplify nanobody genes, generating a high-quality phage-display library of 1.36×10
9
effective clones.
B-N the second round of PCR amplification electrophoresis
Phage Panning:
Two rounds of panning against B-N-BSA enriched high-affinity phage clones, as indicated by strong ELISA signals.
Monoclonal Screening:
192 clones were screened by ELISA, and eight specific nanobody candidates with high binding and low cross-reactivity were identified.
Our Advantages Highlighted:
We would be happy to discuss how we can support your specific project.
Contact Us
Looking for more? These services might be just what you need
:
Protein Expression
Antibody Services
DNA Synthesis
Thank you for connecting with us! Click "
Subscribe
" to receive valuable insights and news. If you prefer not to receive further emails, just hit "
Unsubscribe
" — your choice is always respected.
Synbio Technologies LLC
9 Deer Prk Dr., Suite J-25, Monmouth Junction, NJ 08852, USA